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A simple vacuum transfer device for nucleic acids.

作者信息

Bignon C, Roux M, Martin P M

机构信息

Laboratoire de Cancérologie Expérimentale, Faculté de Médecine Nord, Marseille, France.

出版信息

J Biochem Biophys Methods. 1992 Oct;25(2-3):185-7. doi: 10.1016/0165-022x(92)90010-8.

DOI:10.1016/0165-022x(92)90010-8
PMID:1491103
Abstract

We present a new vacuum device intended for transferring nucleic acids from agarose gels onto hybridization membranes. The apparatus is drawn so that plastic sheets serve as substitutes for the elaborate, but cumbersome and unnecessary, locking systems mounted on all the commercial blotters. This substitution results in (1) a much more easy and rapid set-up of the transfer and (2) a very simple framework allowing the possibility of low-cost manufacturing.

摘要

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