通过薄层色谱法从表雄酮中分离雄酮，以及从其3-羟基差向异构体中分离脱氢表雄酮。
Separation of androsterone from epiandrosterone, and dehydroepiandrosterone from its 3-hydroxy epimer by thin-layer chromatography.
作者信息
Tinschert W, Träger L
出版信息
J Chromatogr. 1978 May 21;152(2):447-52. doi: 10.1016/s0021-9673(00)85081-5.
The application of thin-layer chromatography to the separation of 13 steroids, including androstanes, 4-androstenes and 5-androstenes, using silica gel and 1,2-propanediol-impregnated cellulose is described. After group-wise separation of various C19 steroids on silica gel, the 3-hydroxy epimers of 5alpha-androstanes and 5-androstenes can be separated by thin-layer chromatography on impregnated cellulose plates. The chromatographic procedure is rapid and makes the prior formation of steroid derivatives unnecessary.
本文描述了使用硅胶和1,2 - 丙二醇浸渍纤维素，通过薄层色谱法分离13种甾体化合物，包括雄烷、4 - 雄烯和5 - 雄烯。在硅胶上对各种C19甾体进行分组分离后，5α - 雄烷和5 - 雄烯的3 - 羟基差向异构体可通过浸渍纤维素板上的薄层色谱法进行分离。该色谱方法快速，无需预先形成甾体衍生物。
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