Kaname S
Fourth Department of Internal Medicine, University of Tokyo Branch Hospital.
Nihon Rinsho. 1992 Dec;50(12):3027-31.
TGF-beta recently has been shown to inhibit mesangial cell growth and to stimulate mesangial matrix synthesis by mesangial cells. Cultured rat mesangial cells expressed 2.5 kb TGF-beta mRNA, and removal of fetal calf serum (FCS) for two days decreased the TGF-beta mRNA level, which was then stimulated by addition of 17% FCS and TPA, one of the phorbol esters, although it is also reported by others that the mRNA expression was stimulated by PDGF, EGF, or high glucose. Bioassay and immunoblot analysis showed that mesangial cells produce and secrete substantial amounts of TGF-beta but mostly in latent forms. Moreover, addition of anti-TGF-beta neutralizing antibodies augmented mesangial cell growth, indicating that the secreted TGF-beta exerts a growth-inhibitory action on themselves. Thus, TGF-beta may function as an autocrine factor in mesangial cells, and it is suggested that mesangial cells may play an important role in the pathogenesis of glomerulopathy.
近来研究表明，转化生长因子-β（TGF-β）可抑制系膜细胞生长，并刺激系膜细胞合成系膜基质。培养的大鼠系膜细胞表达2.5 kb的TGF-β mRNA，去除胎牛血清（FCS）两天可降低TGF-β mRNA水平，随后添加17% FCS和佛波酯之一的TPA可刺激该水平升高，不过也有其他人报道，血小板衍生生长因子（PDGF）、表皮生长因子（EGF）或高糖可刺激mRNA表达。生物活性测定和免疫印迹分析表明，系膜细胞产生并分泌大量TGF-β，但大多以潜伏形式存在。此外，添加抗TGF-β中和抗体可增强系膜细胞生长，这表明分泌的TGF-β对其自身发挥生长抑制作用。因此，TGF-β可能作为系膜细胞中的自分泌因子发挥作用，提示系膜细胞可能在肾小球病发病机制中起重要作用。
