Yao J, Li L S
Department of Nephrology, Jinling Hospital, Nanjing.
Chin Med J (Engl). 1994 Jun;107(6):455-9.
The present study was designed to test the hypothesis that the inhibitory effect of elevated glucose levels on mesangial cell growth might be mediated by transforming growth factor beta (TGF beta). Increased glucose levels inhibited mesangial cell proliferation in a concentration dependent manner. The addition of a rabbit antipocine TGF beta 1 neutralizing antibody significantly enhanced the cell proliferation in both normal (5.5 mM) and high (50 mM) glucose media (12% and 30% respectively), and almost completely blocked the growth inhibition induced by high glucose media. TGF beta assay demonstrated mesangial cells produced more active than latent TGF beta after growth in high glucose media. We conclude that TGF beta functions as an autocrine cytokine in mesangial cells growth and the production of TGF beta is modulated by high glucose concentration. The growth inhibition induced by high glucose levels may be largely mediated by endogenous TGF beta.
血糖水平升高对系膜细胞生长的抑制作用可能是由转化生长因子β（TGF-β）介导的。血糖水平升高以浓度依赖的方式抑制系膜细胞增殖。添加兔抗猪TGF-β1中和抗体可显著增强正常（5.5 mM）和高糖（50 mM）培养基中细胞的增殖（分别为12%和30%），并几乎完全阻断高糖培养基诱导的生长抑制。TGF-β检测表明，系膜细胞在高糖培养基中生长后产生的活性TGF-β比潜伏性TGF-β更多。我们得出结论，TGF-β在系膜细胞生长中作为一种自分泌细胞因子发挥作用，且TGF-β的产生受高糖浓度调节。高糖水平诱导的生长抑制可能很大程度上是由内源性TGF-β介导的。
