Obata T, Yamanaka Y, Chiueh C C
Department of Pharmacology, Oita Medical University, Japan.
Jpn J Pharmacol. 1992 Nov;60(3):311-3. doi: 10.1254/jjp.60.311.
We examined the effects of 1-methyl-4-phenylpyridinium ion (MPP+) on the release of DA in rat striatum by the in vivo microdialysis technique. For this study, we made a suitable microdialysis probe from a 22-G needle, microliter pipette tip, silica tube and polyethylene tube. Such a repairable microdialysis probe can be easily made from readily available and inexpensive materials. DA release, as determined by the 3-methoxytyramine level, was dose-dependently increased by MPP+ (1-10 mM). Only the presence of a 1 mM concentration of MPP+ in the dialysate significantly decreased the level of the DA metabolite DOPAC, while administration of higher MPP+ concentrations resulted in no significant change.
我们采用体内微透析技术研究了1-甲基-4-苯基吡啶离子（MPP+）对大鼠纹状体中多巴胺（DA）释放的影响。在本研究中，我们用一根22G的针、微量移液器吸头、硅胶管和聚乙烯管制作了一个合适的微透析探针。这种可修复的微透析探针可以很容易地用现成且便宜的材料制成。由3-甲氧基酪胺水平测定的DA释放量随MPP+（1-10 mM）浓度的增加而呈剂量依赖性增加。只有透析液中存在1 mM浓度的MPP+时，DA代谢产物3,4-二羟基苯乙酸（DOPAC）的水平才会显著降低，而给予更高浓度的MPP+则无显著变化。
