Yan G, Fukabori Y, Nikolaropoulos S, Wang F, McKeehan W L
W. Alton Jones Cell Science Center, Inc. Lake Placid, New York 12946.
Mol Endocrinol. 1992 Dec;6(12):2123-8. doi: 10.1210/mend.6.12.1491693.
The growth of isolated epithelial and stromal cells from both androgen-dependent normal rat prostate and an androgen-responsive model rat prostate tumor is androgen-independent. When added to co-cultures of epithelial and stromal cells separated by a semipermeable membrane, androgen stimulated epithelial cell growth without an effect on stromal cell growth. Northern blot and nuclease protection analysis of mRNA revealed that stromal cells specifically expressed an androgen-sensitive secreted member of the heparin-binding fibroblast growth factor family [keratinocyte growth factor (KGF)/fibroblast growth factor-7]. KGF was mitogenic for epithelial cells, but not for stromal cells. Epithelial cells expressed specifically a splice variant of the bek receptor gene that specifically binds KGF. Expression of the bek receptor gene in stromal cells was undetectable by Northern blot and nuclease protection analyses. The results suggest that stromal cell-derived KGF has the properties of an andromedin, which mediates the indirect control of epithelial cell proliferation by androgen through a directional stromal-to-epithelial cell paracrine mechanism.
从雄激素依赖的正常大鼠前列腺和雄激素反应性模型大鼠前列腺肿瘤中分离出的上皮细胞和基质细胞的生长是雄激素非依赖性的。当添加到由半透膜分隔的上皮细胞和基质细胞共培养物中时，雄激素刺激上皮细胞生长，而对基质细胞生长没有影响。对mRNA进行Northern印迹和核酸酶保护分析表明，基质细胞特异性表达肝素结合成纤维细胞生长因子家族的一种雄激素敏感分泌成员[角质形成细胞生长因子（KGF）/成纤维细胞生长因子-7]。KGF对上皮细胞有促有丝分裂作用，但对基质细胞没有作用。上皮细胞特异性表达bek受体基因的一种剪接变体，该变体特异性结合KGF。通过Northern印迹和核酸酶保护分析未检测到基质细胞中bek受体基因的表达。结果表明，基质细胞衍生的KGF具有雄激素介质的特性，它通过定向的基质细胞到上皮细胞旁分泌机制介导雄激素对上皮细胞增殖的间接控制。
