Harada M, Shimizu A, Nakamura Y, Nemoto R
Laboratory of Pathology, Kanagawa Cancer Center Research Institute, Yokohama, Japan.
Adv Exp Med Biol. 1992;324:83-92. doi: 10.1007/978-1-4615-3398-6_8.
Bromodeoxyuridine (BrdU) labeled human prostatic cancer cells, PC-3, and murine osteosarcoma cells, POS-1 were injected into the tail veins of male mice under concomitant temporal occlusion of inferior vena cava. Five minutes after release of the venous occlusion, animals were sacrificed and various tissues, organs and the vertebral bones were examined immunohistochemically using an application of BrdU-anti-BrdU methods. Obvious BrdU labeled tumor cells, isolated or clumped, were demonstrated within the venous channels along the vertebral column, the epidural venous channels around spinal nervous tissues, in the bone marrow of lumbo-sacral vertebrae and intra- and peri-prostatic venous channels. The results suggest that a blockade of short duration of venous flow at the inferior vena cava can result in the bypassing of tumor cells through the vena cava to the vertebral venous system, which has a close connection with the peri-prostatic venous plexus. Thus, the vertebral venous system may play an important role in the metastasis of prostatic carcinoma to bone. In addition this experimental procedure is a very valuable model for studying mechanisms and prevention of bone metastases from prostatic carcinoma.
将溴脱氧尿苷（BrdU）标记的人前列腺癌细胞PC-3和小鼠骨肉瘤细胞POS-1注入雄性小鼠尾静脉，同时暂时阻断下腔静脉。静脉阻塞解除5分钟后，处死动物，使用BrdU-抗BrdU方法对各种组织、器官和椎骨进行免疫组织化学检查。在沿脊柱的静脉通道、脊髓神经组织周围的硬膜外静脉通道、腰骶椎骨髓以及前列腺内和前列腺周围静脉通道中，均发现了明显的BrdU标记的肿瘤细胞，这些细胞呈单个或聚集状。结果表明，下腔静脉短时间的血流阻断可导致肿瘤细胞经腔静脉绕过，进入与前列腺周围静脉丛密切相关的椎静脉系统。因此，椎静脉系统可能在前列腺癌骨转移中起重要作用。此外，该实验方法是研究前列腺癌骨转移机制及预防的非常有价值的模型。
