MILLER Z B, WALDMAN J, McLEAN F C
J Exp Med. 1952 May 1;95(5):497-508. doi: 10.1084/jem.95.5.497.
The calcification of rat hypertrophic cartilage slices in vitro is markedly inhibited by preliminary exposure to metachromatic and other basic dyes. The dyes are effective at 10(-3) to 10(-4)M in the absence of calcium and phosphate. This inhibition does not occur at the same low dye concentration if calcium and phosphate are present. Neither ion alone is effective in preventing the inhibition. The inhibitory action can be removed by placing slices which have been treated with basic dye in a solution which contains calcium and phosphate ions, plus an acid dye, Orange G. Most acid dyes do not inhibit calcification, except at very high concentrations. Alizarin and quinalizarin are exceptional, and produce marked inhibition at 10(-3)M, an effect which is perhaps attributable to the tendency of these dyes to form lakes with calcium. Rachitic cartilage slices which no longer calcify in inorganic phosphate as a result of treatment with basic dyes show normal calcification in the presence of organic phosphate esters. These results are discussed in terms of the possibility that chondroitin sulfate ester participates in normal calcification.
预先将大鼠肥大软骨切片暴露于异染性染料和其他碱性染料中，可显著抑制其体外钙化。在无钙和磷的情况下，这些染料在10⁻³至10⁻⁴M浓度时有效。如果存在钙和磷，在相同的低染料浓度下则不会发生这种抑制作用。单独的任何一种离子都不能有效防止这种抑制。将用碱性染料处理过的切片置于含有钙和磷酸根离子以及酸性染料橙G的溶液中，可消除抑制作用。除了在非常高的浓度下，大多数酸性染料不会抑制钙化。茜素和喹哪啶茜素是例外，它们在10⁻³M时会产生明显的抑制作用，这种作用可能归因于这些染料与钙形成色淀的倾向。由于用碱性染料处理而不再在无机磷酸盐中钙化的佝偻病软骨切片，在有机磷酸酯存在时显示正常钙化。根据硫酸软骨素酯参与正常钙化的可能性对这些结果进行了讨论。
