Hvidberg A, Jørgensen S, Hilsted J
Department of Internal Medicine and Endocrinology, Hvidovre University Hospital, Copenhagen, Denmark.
Br J Clin Pharmacol. 1992 Dec;34(6):547-50. doi: 10.1111/j.1365-2125.1992.tb05660.x.
To compare the effect on glucose recovery after insulin-induced hypoglycaemia of intramuscular genetically engineered glucagon, intramuscular glucagon from pancreatic extraction and intravenous glucose, we examined 10 healthy subjects during blockage of glucose counterregulation with somatostatin, propranolol and phentolamine. Each subject was studied on three separate occasions. Thirty min after a bolus injection of 0.075 iu soluble insulin per kilogram body weight the subjects received one of the following treatments: 1 mg glucagon from pancreatic extraction intramuscularly; 1 mg genetically engineered glucagon intramuscularly; and 25 g glucose intravenously, respectively. The two glucagon preparations induced an equally rapid increase in plasma glucose. This was due to an abrupt (within 4 min) and equal increase in glucose appearance rate. The increases in both plasma glucose and in glucose appearance rate were far more protracted after i.m. glucagon than after i.v. glucose. These results suggest that genetically engineered glucagon and glucagon from pancreatic extraction have a similar effect on hepatic glucose production rate. Due to the protracted effect of intramuscular glucagon, a combined treatment consisting of both intravenous glucose and intramuscular glucagon may be more effective in the treatment of hypoglycaemia than any of these given alone.
为比较基因工程胰高血糖素肌肉注射、胰腺提取物肌肉注射胰高血糖素及静脉注射葡萄糖对胰岛素诱导低血糖后血糖恢复的影响，我们在使用生长抑素、普萘洛尔和酚妥拉明阻断葡萄糖反向调节期间，对10名健康受试者进行了研究。每位受试者在三个不同的时间接受研究。在按每公斤体重静脉推注0.075国际单位可溶性胰岛素30分钟后，受试者分别接受以下一种治疗：1毫克胰腺提取物胰高血糖素肌肉注射；1毫克基因工程胰高血糖素肌肉注射；以及25克葡萄糖静脉注射。两种胰高血糖素制剂均使血糖迅速升高。这是由于葡萄糖出现率突然（4分钟内）且同等程度地增加。肌肉注射胰高血糖素后，血糖和葡萄糖出现率的升高比静脉注射葡萄糖后更为持久。这些结果表明，基因工程胰高血糖素和胰腺提取物胰高血糖素对肝脏葡萄糖生成率有相似的作用。由于肌肉注射胰高血糖素的作用持久，静脉注射葡萄糖和肌肉注射胰高血糖素联合治疗低血糖可能比单独使用任何一种治疗方法更有效。
