C-terminal amidation of calcitonin by carboxypeptidase Y catalyzed transpeptidation with a photocleavable nucleophile.
作者信息
Henriksen D B, Rolland M, Jakobsen M H, Buchardt O, Breddam K
机构信息
University of Copenhagen, Denmark.
出版信息
Pept Res. 1992 Nov-Dec;5(6):321-4.
PMID:1493358
Abstract
The C-terminal amidation of calcitonin represents an important technological problem. A method using a serine carboxypeptidase-catalyzed transpeptidation reaction in combination with photochemical cleavage to give the warranted peptide amide is described. The overall yield is higher than 95%.
摘要
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