Galla T J, Anton-Lamprecht I, Kieser M, Saetzler R K, Messmer K
Department of Experimental Surgery, University of Heidelberg, Germany.
Br J Plast Surg. 1992 Nov-Dec;45(8):578-85. doi: 10.1016/0007-1226(92)90023-q.
In random pattern skin flaps of mice, tissue fluorescence measured by means of videodensitometry at 24 h after flap elevation significantly correlates with intravital microscopically measured functional vessel density and viability of skin tissue as assessed by transmission light and electron microscopy. The correlation was found reproducible in non-ischaemic flaps (r = 0.86) and flaps being rendered ischaemic for 6 h after elevation (r = 0.98), indicating that increased microvascular permeability as a result of ischaemia/reperfusion does neither affect tissue fluorescence nor the accuracy of the fluorescein dye test. In addition, tissue fluorescence at 24 h after flap elevation accurately predicts ultimate flap survival on the 7th postoperative day with a sensitivity of 0.89 and a specificity of 0.85. These results suggest that in non-ischaemic as well as in ischaemic random pattern skin flaps tissue fluorescence can be used for assessment of nutritive blood flow, viability of skin tissue and ultimate tissue survival in these flaps.
在小鼠随意型皮瓣中，皮瓣掀起后24小时通过视频密度测定法测得的组织荧光与活体显微镜下测量的功能性血管密度以及通过透射光和电子显微镜评估的皮肤组织活力显著相关。在非缺血性皮瓣（r = 0.86）和掀起后缺血6小时的皮瓣（r = 0.98）中发现这种相关性具有可重复性，这表明缺血/再灌注导致的微血管通透性增加既不影响组织荧光，也不影响荧光素染料试验的准确性。此外，皮瓣掀起后24小时的组织荧光能够准确预测术后第7天皮瓣的最终存活情况，敏感性为0.89，特异性为0.85。这些结果表明，在非缺血性以及缺血性随意型皮瓣中，组织荧光可用于评估这些皮瓣的营养血流、皮肤组织活力和最终组织存活情况。
