Mutvei A, Dihlmann S, Herth W, Hurt E C
European Molecular Biology Laboratory, Heidelberg/Germany.
Eur J Cell Biol. 1992 Dec;59(2):280-95.
The essential yeast nuclear pore protein NSP1 was placed under the control of the regulatable GAL10 promoter. GAL::NSP1 cells grow normally in galactose medium, but arrest in growth upon glucose-induced repression of the GAL::nsp1 gene. During NSP1 depletion, nuclear accumulation of two reporter proteins Mat alpha 2-lacZ and PHO2-lacZ is inhibited, and the chimeric proteins appear in the cytoplasm of GAL::nsp1 cells. Furthermore, the nuclear pore density decreases within the nuclear membrane during early NSP1 depletion. Upon reinduction of the NSP1 gene after NSP1 depletion, NSP1 is targeted to the nuclear envelope, the nuclear pore density increases, and nuclear accumulation of reporter proteins is restored.
酵母必需的核孔蛋白NSP1受可调控的GAL10启动子控制。GAL::NSP1细胞在半乳糖培养基中正常生长，但在葡萄糖诱导的GAL::nsp1基因抑制下生长停滞。在NSP1缺失期间，两种报告蛋白Matα2-lacZ和PHO2-lacZ的核积累受到抑制，并且嵌合蛋白出现在GAL::nsp1细胞的细胞质中。此外，在NSP1早期缺失期间，核膜内的核孔密度降低。在NSP1缺失后重新诱导NSP1基因时，NSP1靶向核膜，核孔密度增加，报告蛋白的核积累得以恢复。
