Department of Biology, Temple University, Philadelphia, Pennsylvania, USA.
Bioessays. 2022 Aug;44(8):e2200027. doi: 10.1002/bies.202200027. Epub 2022 Jun 26.
The nuclear export of mRNA through the nuclear pore complex (NPC) is a process required for the healthy functioning of human cells, making it a critical area of research. However, the geometries of mRNA and the NPC are well below the diffraction limit of light microscopy, thereby presenting significant challenges in evaluating the discrete interactions and dynamics involved in mRNA nuclear export through the native NPC. Recent advances in biotechnology and single-molecule super-resolution light microscopy have enabled researchers to gain granular insight into the specific contributions made by discrete nucleoporins in the nuclear basket of the NPC to the export of mRNA. Specifically, by expanding upon the docking step facilitated by the protein TPR in the nuclear basket as well as identifying NUP153 as being the primary nuclear basket protein initiating export through the central channel of the NPC.
mRNA 通过核孔复合体（NPC）的核输出是人类细胞正常功能所必需的过程，因此成为了一个关键的研究领域。然而，mRNA 和 NPC 的几何形状远低于光显微镜的衍射极限，从而在评估通过天然 NPC 进行的 mRNA 核输出的离散相互作用和动力学方面带来了重大挑战。生物技术和单分子超分辨率荧光显微镜的最新进展使研究人员能够深入了解 NPC 核篮状结构中的离散核孔蛋白在 mRNA 核输出中所做出的具体贡献。具体来说，通过扩展核篮状结构中 TPR 蛋白所促进的对接步骤，并确定 NUP153 是通过 NPC 中央通道起始输出的主要核篮状结构蛋白。
