Biljanović-Paunović L, Stojanović N, Mostarica-Stojković M, Ramić Z, Ivanović Z, Basara N, Clemons G K, Pavlović-Kentera V
Institute for Medical Research, Belgrade, Yugoslavia.
Exp Hematol. 1992 Dec;20(11):1257-62.
In this study, the extent to which growth factor production and microenvironment might be responsible for defective erythropoiesis and granulopoiesis in anemic b/b rats is investigated. Radioimmunoassay-determined serum erythropoietin (Epo) levels are high in b/b rats and closely related to degree of anemia. The low number of erythroid progenitors in b/b rats despite a high Epo level suggested that the defective erythropoiesis could be due to a low level of burst-promoting activity (BPA). A pokeweed mitogen-stimulated medium (PWM-SCM) was prepared with b/b rat spleen cells and used in normal and anemic rat bone marrow and spleen cultures to determine BPA and other growth factor levels. No erythroid burst-forming unit-derived colonies were found but granulocyte-macrophage colony-forming units were counted in significant number, suggesting that the production of growth factors that supports the growth of granulopoietic progenitors is not significantly disturbed. Because BPA is produced mainly by T-lymphocytes, the low BPA level in b/b rat PWM-SCM raised the question of the functional capacity of T-lymphocytes. Investigations showed a decrease in the proliferative activity of b/b rat spleen mitogen-activated T-lymphocytes to about 20% of controls as well as a decrease in interleukin-2 activity in b/b rat spleen cell supernatants. These results point to defective T-lymphocytes. A study of bone marrow fibroblastoid cell colonies (CFU-F) revealed significantly lower CFU-F counts in the b/b rats. This finding is indicative of a disturbed microenvironment, which could also to some extent be responsible for decreased growth factor production and depressed hematopoiesis in the b/b rat.
在本研究中，探讨了生长因子产生和微环境在贫血b/b大鼠红细胞生成和粒细胞生成缺陷中可能所起的作用程度。放射免疫分析测定的b/b大鼠血清促红细胞生成素（Epo）水平较高，且与贫血程度密切相关。尽管Epo水平较高，但b/b大鼠中红系祖细胞数量较少，提示红细胞生成缺陷可能是由于爆式促进活性（BPA）水平较低所致。用b/b大鼠脾细胞制备商陆有丝分裂原刺激培养基（PWM-SCM），并用于正常和贫血大鼠的骨髓及脾细胞培养，以测定BPA和其他生长因子水平。未发现源自红系爆式形成单位的集落，但计数到大量粒细胞-巨噬细胞集落形成单位，这表明支持粒细胞生成祖细胞生长的生长因子产生未受到明显干扰。由于BPA主要由T淋巴细胞产生，b/b大鼠PWM-SCM中BPA水平较低，这引发了T淋巴细胞功能能力的问题。研究表明，b/b大鼠脾有丝分裂原激活的T淋巴细胞增殖活性降至对照组的约20%，同时b/b大鼠脾细胞上清液中白细胞介素-2活性也降低。这些结果表明T淋巴细胞存在缺陷。对骨髓成纤维样细胞集落（CFU-F）的研究显示，b/b大鼠的CFU-F计数显著降低。这一发现表明微环境受到干扰，这在一定程度上也可能是b/b大鼠生长因子产生减少和造血功能受抑制的原因。
