Bastien P, Killick-Kendrick R
Department of Biology, Imperial College of Science, Technology and Medicine, Ascot, Berkshire, U.K.
Exp Parasitol. 1992 Dec;75(4):433-41. doi: 10.1016/0014-4894(92)90256-a.
Four strains of Leishmania tropica s. st. from Afghanistan were found to be constantly infective to the golden hamster (Mesocricetus auratus), whether inoculated as culture promastigotes from early passages or as amastigotes from the cutaneous lesions. In total, 66 amastigote passages were carried out over 6 passage generations. The footpad lesions were chronic and nonulcerative infiltrates; their evolution is described up to 15 months. No self-healing was observed. Balb/c mice and gerbils were not susceptible. Earlier studies on L. tropica s. st. in laboratory animals, principally by Soviet and French workers, are critically reviewed. This new experimental model of chronic cutaneous leishmaniasis is of special interest for studies on the immunology and chemotherapy of the disease. Some advantages over the commonly used L. major model are discussed.
从阿富汗分离出的4株热带利什曼原虫指名亚种，无论是接种早期传代培养的前鞭毛体，还是接种来自皮肤病变部位的无鞭毛体，均发现对金黄仓鼠（Mesocricetus auratus）具有持续感染性。总共进行了6代66次无鞭毛体传代。足垫病变为慢性非溃疡性浸润；描述了其长达15个月的演变过程。未观察到自愈现象。Balb/c小鼠和沙鼠不易感。对早期主要由苏联和法国研究人员进行的关于热带利什曼原虫指名亚种在实验动物中的研究进行了批判性综述。这种新的慢性皮肤利什曼病实验模型对于该疾病的免疫学和化疗研究具有特殊意义。讨论了相对于常用的硕大利什曼原虫模型的一些优势。
