用薄层聚丙烯酰胺凝胶等电聚焦法微量测定人红细胞中葡萄糖-6-磷酸脱氢酶同工酶活性
Microdetermination of G6PD isoenzyme activity in human erythrocytes by thin-layer PAG-IEF.
作者信息
Nie C, Zhao S, Lu Y
机构信息
Hunan Medical University, Changsha, China.
出版信息
J Biochem Biophys Methods. 1992 Dec;25(4):245-51. doi: 10.1016/0165-022x(92)90019-7.
An improved method of microdetermination of G6PD isoenzyme activity in human erythrocytes was developed by modification of previously reported procedures. The volume of blood samples was reduced from 2 ml to 20 microliters. After hemolysis in 2% Triton X-100 and 0.1% beta-mercaptoethanol, the samples were subjected to centrifugation and thin-layer isoelectric focusing in polyacrylamide gel (PAG-IEF). By comparison with the original method, excellent resolution was obtained by this more rapid and simple procedure.
通过改进先前报道的方法，开发出一种改进的微量测定人红细胞中G6PD同工酶活性的方法。血样体积从2毫升减少到20微升。在2% Triton X-100和0.1% β-巯基乙醇中溶血后，样品进行离心并在聚丙烯酰胺凝胶中进行薄层等电聚焦（PAG-IEF）。与原方法相比，这种更快速、简单的方法获得了出色的分辨率。
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