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[检测和测定含氮霉菌毒素的方法]

[Methods of detecting and determining nitrogen-containing mycotoxins].

作者信息

Scott P M

出版信息

Prikl Biokhim Mikrobiol. 1992 Nov-Dec;28(6):819-27.

PMID:1494569
Abstract

The influence of various nitrogen functional groups used for extraction cleanup and determination of N-containing mycotoxins (NM) in feeds and foodstuffs have been considered. TLC and LC are the most common techniques for detection and determination of nitrogen-containing mycotoxins. Gas chromatography has been used for determination (with or without derivatization) of several nitrogen-containing mycotoxins and/or their degradation products. Immunochemical techniques, in particular ELISA are available for only a very limited number of NM (e.g. ochratoxin A). Numerous methods for determination of ochratoxin A in feeds, grains, animal products and other foodstuffs have been developed. Methods for which recoveries have been carried out on spiked samples are also available for several other NM.

摘要

已考虑了用于饲料和食品中含氮霉菌毒素(NM)提取净化和测定的各种氮官能团的影响。薄层色谱法(TLC)和液相色谱法(LC)是检测和测定含氮霉菌毒素最常用的技术。气相色谱法已用于几种含氮霉菌毒素和/或其降解产物的测定(有或没有衍生化)。免疫化学技术,特别是酶联免疫吸附测定法(ELISA)仅适用于极少数的含氮霉菌毒素(如赭曲霉毒素A)。已开发出许多用于测定饲料、谷物、动物产品和其他食品中赭曲霉毒素A的方法。对加标样品进行回收率测定的方法也适用于其他几种含氮霉菌毒素。

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