Sinel'shchikova T A, Zasukhina G D
Radiobiologiia. 1992 Nov-Dec;32(6):826-9.
The human fibroblasts were gamma-irradiated with low doses (0.07-0.21 Gy). After a short time interval (3 h), a study was made of the postirradiation viability of cells (by the trypan blue dye exclusion method); post-N-methyl-N'-nitro-nitrosoguanidine-DNA synthesis (by 3H-thymidine incorporation immediately after the mutagen treatment) and postirradiation induction of DNA single-strand breaks (by alkaline elution of cells lysed on the membrane filters). The preirradiation of cells with low doses of gamma-rays was shown to render the cells resistance to induction of DNA breaks by the following exposure to gamma-radiation. The survival rate increased; DNA synthesis was resistant to alkylation damage in these cells, as compared to nonirradiated cells.
将人类成纤维细胞用低剂量（0.07 - 0.21戈瑞）的γ射线进行照射。在短时间间隔（3小时）后，对细胞照射后的活力（通过台盼蓝染料排斥法）、照射后N - 甲基 - N'-硝基 - 亚硝基胍处理后的DNA合成（诱变处理后立即通过3H - 胸腺嘧啶核苷掺入法）以及照射后DNA单链断裂的诱导情况（通过对膜滤器上裂解的细胞进行碱性洗脱法）进行了研究。结果表明，用低剂量γ射线对细胞进行预照射可使细胞对随后照射γ射线诱导的DNA断裂产生抗性。与未照射的细胞相比，这些细胞的存活率增加；DNA合成对烷基化损伤具有抗性。
