Xia Y, Goebel D J, Kapatos G, Bannon M J
Department of Psychiatry, Wayne State University School of Medicine, Detroit, Michigan.
J Neurochem. 1992 Sep;59(3):1179-82. doi: 10.1111/j.1471-4159.1992.tb08365.x.
Dopamine transporter mRNA levels in the rat substantia nigra were quantified using a sensitive nuclease protection assay with a highly homologous human dopamine transporter cDNA clone. The same probe was also used to visualize dopamine transporter mRNA in the substantia nigra by in situ hybridization. Repeated cocaine administration (15 mg/kg, twice a day for 6.5 days) resulted in a greater than 40% decrease in nigral dopamine transporter mRNA levels. In contrast, dopamine transporter mRNA levels were unchanged after either acute treatment (4 h before death) or repeated cocaine treatment followed by a 72-h withdrawal period. Thus, blockade of the dopamine transporter by repeated cocaine administration may result in the down-regulation of dopamine transporter gene expression in dopamine neurons.
使用一种灵敏的核酸酶保护分析法，采用高度同源的人类多巴胺转运体cDNA克隆，对大鼠黑质中的多巴胺转运体mRNA水平进行定量分析。同一探针也用于通过原位杂交观察黑质中的多巴胺转运体mRNA。反复给予可卡因（15毫克/千克，每天两次，共6.5天）导致黑质中多巴胺转运体mRNA水平下降超过40%。相比之下，急性处理（死亡前4小时）或反复给予可卡因后再经过72小时戒断期，多巴胺转运体mRNA水平未发生变化。因此，反复给予可卡因阻断多巴胺转运体可能导致多巴胺能神经元中多巴胺转运体基因表达的下调。
