热休克对抗生素选择下转基因冈比亚按蚊（双翅目：蚊科）存活的影响

Effects of heat shock on the survival of transgenic Anopheles gambiae (Diptera: Culicidae) under antibiotic selection.

作者信息

Sakai R K, Miller L H

机构信息

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

J Med Entomol. 1992 Mar;29(2):374-5. doi: 10.1093/jmedent/29.2.374.

DOI:10.1093/jmedent/29.2.374

PMID:1495063

Abstract

A gene for neomycin resistance linked to a Drosophila hsp 70 heat shock promoter was introduced into the germ line of Anopheles gambiae Giles. Effects of heat shock at 37 and 41 degrees C on the subsequent survival of the transgenic mosquito subjected to selection by the antibiotic G418 were studied. Heat shock did not enhance the survival of untransformed mosquitoes but greatly increased the survival of the transgenic mosquitoes. Survival after heat shock at 41 degrees C was greater than after heat shock at 37 degrees C.

摘要

一个与果蝇热休克蛋白70（hsp 70）热休克启动子相连的新霉素抗性基因被导入冈比亚按蚊（Anopheles gambiae Giles）的种系中。研究了37℃和41℃热休克对经抗生素G418筛选的转基因蚊子后续存活情况的影响。热休克并未提高未转化蚊子的存活率，但极大地提高了转基因蚊子的存活率。41℃热休克后的存活率高于37℃热休克后的存活率。