Kocan A A, Mukolwe S W, Murphy G L, Barker R W, Kocan K M
College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
J Med Entomol. 1992 Jul;29(4):630-3. doi: 10.1093/jmedent/29.4.630.
Borrelia burgdorferi was isolated from Ixodes scapularis Say and Dermacentor albipictus Packard that were removed as partially fed adults from white-tailed deer, Odocoileus virginianus Zimmermann, in Oklahoma. Isolation in media was accomplished only after homogenates of pooled field-collected ticks were inoculated into laboratory-reared Peromyscus leucopus and reisolated from the urinary bladder into BSK II media. Both isolates were confirmed by western blot analysis and reactivity with monoclonal antibody H5332. These are the first reported isolates of B. burgdorferi from Oklahoma from these two tick species and are the first isolates from ticks from the south-central United States that were infective for laboratory-reared P. leucopus.
从肩突硬蜱（Ixodes scapularis Say）和白纹革蜱（Dermacentor albipictus Packard）中分离出伯氏疏螺旋体（Borrelia burgdorferi），这些蜱是从俄克拉荷马州的白尾鹿（Odocoileus virginianus Zimmermann）身上采集的部分饱血成虫。只有在将野外采集的蜱虫匀浆接种到实验室饲养的白足鼠（Peromyscus leucopus）中，并从膀胱中重新分离到BSK II培养基中后，才能在培养基中实现分离。两种分离株均通过蛋白质印迹分析以及与单克隆抗体H5332的反应性得到确认。这些是首次报道的来自俄克拉荷马州这两种蜱虫物种的伯氏疏螺旋体分离株，也是首次从美国中南部对实验室饲养的白足鼠具有感染性的蜱虫中分离出的菌株。
