Gill J S, Johnson R C, Sinclair M K, Weisbrod A R
Department of Microbiology, School of Medicine, University of Minnesota, Minneapolis 55455.
J Wildl Dis. 1993 Jan;29(1):64-72. doi: 10.7589/0090-3558-29.1.64.
During a special two-day hunt (11, 12 November 1989) in Saint Croix State Park, Minnesota (USA), one side of the neck for each of 146 white-tailed deer (Odocoileus virginianus) was examined for ticks. Of the 5,442 ticks collected, 90% (4,893) were the winter tick, Dermacentor albipictus, and 10% (549) were the deer tick, Ixodes dammini, the primary vector of the causative agent of Lyme disease, Borrelia burgdorferi. Adult males had the greatest frequency of infestation of either D. albipictus (100%) or I. dammini (88%) and had on average more ticks, compared to other deer. Based on an examination of midgut material from 435 I. dammini by polyclonal antibody analysis, spirochetes were observed in 22% of the ticks. Species-specific monoclonal antibody analysis of the spirochetes confirmed that the bacteria were B. burgdorferi.
1989年11月11日至12日，在美国明尼苏达州的圣克罗伊州立公园进行了一次为期两天的特别狩猎活动，检查了146只白尾鹿（弗吉尼亚鹿）颈部一侧的蜱虫。在采集到的5442只蜱虫中，90%（4893只）是冬季蜱（白纹革蜱），10%（549只）是鹿蜱（达氏硬蜱），后者是莱姆病病原体伯氏疏螺旋体的主要传播媒介。成年雄性白尾鹿感染白纹革蜱（100%）或达氏硬蜱（88%）的频率最高，且与其他鹿相比，平均身上的蜱虫更多。通过多克隆抗体分析对435只达氏硬蜱的中肠物质进行检测，在22%的蜱虫中发现了螺旋体。对这些螺旋体进行物种特异性单克隆抗体分析，证实这些细菌为伯氏疏螺旋体。
