Twumasi D Y, Liener I E
J Biol Chem. 1977 Mar 25;252(6):1917-26.
A procedure is described for the purification of the elastase and chymotrypsin-like enzymes from purulent sputum. This procedure permitted the isolation of 132 mg and 120 mg of the elastase and chymotrypsin-like enzymes, respectively, from 230 g of purulent sputum. The elastase enzymes consist of a family of five isozymes, and at least three isozymes comprise the chymotrypsin-like enzyme system. The elastases proved to be immunologically identical with the corresponding enzyme of human leukocytes. These enzymes were characterized with respect to molecular weight, amino acid and carbohydrate composition, several kinetic parameters, and inhibition by various synthetic and natural inhibitors. The properties so found were comparable to those which had been previously reported by others for the elastase and chymotrypsin-like enzymes isolated directly from leukocytic granules.
本文描述了一种从脓性痰中纯化弹性蛋白酶和类胰凝乳蛋白酶的方法。该方法从230克脓性痰中分别分离出了132毫克弹性蛋白酶和120毫克类胰凝乳蛋白酶。弹性蛋白酶由五种同工酶组成的家族，类胰凝乳蛋白酶系统至少由三种同工酶组成。已证明弹性蛋白酶在免疫学上与人白细胞的相应酶相同。对这些酶进行了分子量、氨基酸和碳水化合物组成、几个动力学参数以及各种合成和天然抑制剂抑制作用等方面的表征。所发现的这些特性与其他人先前报道的直接从白细胞颗粒中分离出的弹性蛋白酶和类胰凝乳蛋白酶的特性相当。
