Wax J R, Blakemore K J, Soloski M J, Gibson M, Stetten G
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Obstet Gynecol. 1992 Sep;80(3 Pt 2):533-5.
The cells found in ascites can be processed like amniotic fluid for fetal karyotyping. We have characterized these cells and used them for a rapid cytogenetic result.
Three patients presented with massive fetal ascites detected by sonography. Samples of ascitic fluid were obtained at fetal paracentesis. Cells from the fluid were cultured using standard methods for fetal blood, and were compared with fetal blood lymphocytes and amniocytes. The length of time in culture, chromosome morphology, and mitotic index of ascitic fluid cells were equivalent to those of fetal blood. In the third case, we performed immunophenotyping on the ascitic fluid cells.
Ascitic fluid contains lymphocytes that permit rapid chromosomal analysis within 96 hours.
腹水中发现的细胞可像羊水一样进行处理以进行胎儿核型分析。我们已对这些细胞进行了特征描述，并将其用于快速获得细胞遗传学结果。
三名患者经超声检查发现有大量胎儿腹水。在胎儿穿刺术时获取腹水样本。用标准的胎儿血液培养方法培养腹水中的细胞，并与胎儿血淋巴细胞和羊膜细胞进行比较。腹水细胞的培养时间、染色体形态和有丝分裂指数与胎儿血液的相当。在第三例中，我们对腹水细胞进行了免疫表型分析。
腹水含有淋巴细胞，可在96小时内进行快速染色体分析。
