Drees Jutta, Smith Jason, Schäfer Frank, Steinert Kerstin
Qiagen, Hilden, Germany.
Methods Mol Med. 2004;94:179-90. doi: 10.1385/1-59259-679-7:179.
By using automation and affinity-tag technologies, analysis of the large number of ORFs generated by genome-sequencing projects is greatly accelerated. Protocols describing culture of E. coli in automation-compatible formats and subsequent micro-to large-scale automated purification of 6xHis-tagged proteins are presented.
通过使用自动化和亲和标签技术，基因组测序项目产生的大量开放阅读框的分析得以大大加速。本文介绍了以自动化兼容形式培养大肠杆菌以及随后对6xHis标签蛋白进行微量到大规模自动化纯化的实验方案。
