Reischl Udo
Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
Methods Mol Med. 2004;94:213-24. doi: 10.1385/1-59259-679-7:213.
This chapter describes a straightforward protein purification strategy for the specific separation of insoluble recombinant proteins (so-called "inclusion bodies") located in the E. coli cytoplasm and their subsequent recovery in form of soluble recombinant proteins. Optimization of this technique can overcome in some cases the application of tedious and yield-reducing standard protein purification procedures. Due to the different behavior of individual recombinant proteins during separation, solubilization, and purification, subsequent purification steps may be required to obtain recombinant proteins in such a purity, that they can be used as antigen components of immunological test systems.
本章描述了一种直接的蛋白质纯化策略，用于特异性分离位于大肠杆菌细胞质中的不溶性重组蛋白（所谓的“包涵体”），并随后以可溶性重组蛋白的形式回收它们。在某些情况下，优化该技术可以克服繁琐且降低产量的标准蛋白质纯化程序的应用。由于各个重组蛋白在分离、溶解和纯化过程中的行为不同，可能需要后续的纯化步骤来获得纯度足以用作免疫检测系统抗原成分的重组蛋白。
