• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

串联质谱产生的大规模蛋白质组学数据集的分析、统计验证与传播。

Analysis, statistical validation and dissemination of large-scale proteomics datasets generated by tandem MS.

作者信息

Nesvizhskii Alexey I, Aebersold Ruedi

机构信息

Institute for Systems Biology, 1441 N 34th Street, Seattle, WA 98103, USA.

出版信息

Drug Discov Today. 2004 Feb 15;9(4):173-81. doi: 10.1016/S1359-6446(03)02978-7.

DOI:10.1016/S1359-6446(03)02978-7
PMID:14960397
Abstract

Tandem mass spectrometry has been used increasingly for high-throughput analysis of complex protein samples. A major challenge lies in the consistent, objective and transparent analysis of the large amounts of data generated by such experiments and in their dissemination and publication. Here, we review currently available computational tools and discuss the need for statistical criteria in the analysis of large proteomics datasets.

摘要

串联质谱已越来越多地用于复杂蛋白质样品的高通量分析。一个主要挑战在于对这类实验产生的大量数据进行一致、客观和透明的分析，以及数据的传播和发表。在此，我们综述了目前可用的计算工具，并讨论了在大型蛋白质组学数据集分析中采用统计标准的必要性。

相似文献

1
Analysis, statistical validation and dissemination of large-scale proteomics datasets generated by tandem MS.串联质谱产生的大规模蛋白质组学数据集的分析、统计验证与传播。
Drug Discov Today. 2004 Feb 15;9(4):173-81. doi: 10.1016/S1359-6446(03)02978-7.
2
The analysis of tandem mass spectrometric datasets: high-throughput investigations require high-quality validation.
Drug Discov Today. 2004 May 1;9(9):391-2. doi: 10.1016/S1359-6446(04)03030-2.
3
mProphet: automated data processing and statistical validation for large-scale SRM experiments.mProphet：用于大规模 SRM 实验的自动化数据处理和统计验证。
Nat Methods. 2011 May;8(5):430-5. doi: 10.1038/nmeth.1584. Epub 2011 Mar 20.
4
Analysis and validation of proteomic data generated by tandem mass spectrometry.串联质谱法产生的蛋白质组学数据的分析与验证
Nat Methods. 2007 Oct;4(10):787-97. doi: 10.1038/nmeth1088.
5
VEMS 3.0: algorithms and computational tools for tandem mass spectrometry based identification of post-translational modifications in proteins.VEMS 3.0：用于基于串联质谱法鉴定蛋白质翻译后修饰的算法和计算工具
J Proteome Res. 2005 Nov-Dec;4(6):2338-47. doi: 10.1021/pr050264q.
6
Visualize: a free and open source multifunction tool for proteomics data analysis.可视化：一个免费的开源多功能工具，用于蛋白质组学数据分析。
Proteomics. 2011 Mar;11(6):1058-63. doi: 10.1002/pmic.201000556. Epub 2011 Feb 7.
7
Potential for false positive identifications from large databases through tandem mass spectrometry.通过串联质谱法从大型数据库中产生假阳性鉴定结果的可能性。
J Proteome Res. 2004 Sep-Oct;3(5):1082-5. doi: 10.1021/pr049946o.
8
APP: an Automated Proteomics Pipeline for the analysis of mass spectrometry data based on multiple open access tools.APP：一种基于多个开放获取工具的用于质谱数据分析的自动化蛋白质组学流程。
BMC Bioinformatics. 2014 Dec 30;15(1):441. doi: 10.1186/s12859-014-0441-8.
9
PRIMA: peptide robust identification from MS/MS spectra.
J Bioinform Comput Biol. 2006 Feb;4(1):125-38. doi: 10.1142/s0219720006001746.
10
A multi-model statistical approach for proteomic spectral count quantitation.一种用于蛋白质组学光谱计数定量的多模型统计方法。
J Proteomics. 2016 Jul 20;144:23-32. doi: 10.1016/j.jprot.2016.05.032. Epub 2016 May 31.

引用本文的文献

1
Proteomic analysis of differential expression of lung proteins in response to highly pathogenic avian influenza virus infection in chickens.鸡感染高致病性禽流感病毒后肺组织差异表达蛋白的蛋白质组学分析。
Arch Virol. 2022 Jan;167(1):141-152. doi: 10.1007/s00705-021-05287-5. Epub 2021 Nov 16.
2
A Markov Chain Monte Carlo Method for Estimating the Statistical Significance of Proteoform Identifications by Top-Down Mass Spectrometry.一种基于马尔可夫链蒙特卡罗方法的用于评估通过自上而下质谱法进行蛋白质鉴定的统计显著性的方法。
J Proteome Res. 2019 Mar 1;18(3):878-889. doi: 10.1021/acs.jproteome.8b00562. Epub 2019 Jan 28.
3
A Multivariate Mixture Model to Estimate the Accuracy of Glycosaminoglycan Identifications Made by Tandem Mass Spectrometry (MS/MS) and Database Search.
一种用于估计串联质谱（MS/MS）和数据库搜索进行糖胺聚糖鉴定准确性的多变量混合模型。
Mol Cell Proteomics. 2017 Feb;16(2):255-264. doi: 10.1074/mcp.M116.062588. Epub 2016 Dec 9.
4
Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite .拓展对低等白蚁工蚁和兵蚁木质纤维素分解酶及氧化还原酶的认识
Front Microbiol. 2016 Oct 13;7:1518. doi: 10.3389/fmicb.2016.01518. eCollection 2016.
5
MZDASoft: a software architecture that enables large-scale comparison of protein expression levels over multiple samples based on liquid chromatography/tandem mass spectrometry.MZDASoft：一种软件架构，可基于液相色谱/串联质谱对多个样本的蛋白质表达水平进行大规模比较。
Rapid Commun Mass Spectrom. 2015 Oct 15;29(19):1841-8. doi: 10.1002/rcm.7272.
6
Improving protein identification from tandem mass spectrometry data by one-step methods and integrating data from other platforms.通过一步法改进串联质谱数据中的蛋白质鉴定，并整合来自其他平台的数据。
Brief Bioinform. 2016 Mar;17(2):262-9. doi: 10.1093/bib/bbv043. Epub 2015 Jul 3.
7
GAG-ID: Heparan Sulfate (HS) and Heparin Glycosaminoglycan High-Throughput Identification Software.GAG-ID：硫酸乙酰肝素（HS）和肝素糖胺聚糖高通量鉴定软件。
Mol Cell Proteomics. 2015 Jun;14(6):1720-30. doi: 10.1074/mcp.M114.045856. Epub 2015 Apr 17.
8
Evaluation and optimization of mass spectrometric settings during data-dependent acquisition mode: focus on LTQ-Orbitrap mass analyzers.在数据依赖采集模式下评估和优化质谱设置：重点关注 LTQ-Orbitrap 质量分析仪。
J Proteome Res. 2013 Jul 5;12(7):3071-86. doi: 10.1021/pr3011588. Epub 2013 May 31.
9
Open source libraries and frameworks for mass spectrometry based proteomics: a developer's perspective.基于质谱的蛋白质组学的开源库和框架：开发者视角
Biochim Biophys Acta. 2014 Jan;1844(1 Pt A):63-76. doi: 10.1016/j.bbapap.2013.02.032. Epub 2013 Mar 1.
10
A new approach to evaluating statistical significance of spectral identifications.一种评估光谱鉴定统计显著性的新方法。
J Proteome Res. 2013 Apr 5;12(4):1560-8. doi: 10.1021/pr300453t. Epub 2013 Mar 8.