Dong W, Eichhorn P, Radajewski S, Schleheck D, Denger K, Knepper T P, Murrell J C, Cook A M
Department of Biological Sciences, The University, Konstanz, Germany.
J Appl Microbiol. 2004;96(3):630-40. doi: 10.1111/j.1365-2672.2004.02200.x.
The aims were to test whether Parvibaculum lavamentivoransT degraded commercial linear alkylbenzenesulphonate (LAS) surfactant via omega-oxygenation and beta-oxidation to sulphophenylcarboxylates (SPCs), whether the organism was widespread and reisolable, and whether the degradative community used the 4-sulphocatechol 1,2-dioxygenase to cleave the aromatic ring from LAS.
Heterotrophic P. lavamentivoransT converted LAS (side chain length C10-C13) to SPCs (C4-C13), alpha,beta-unsaturated SPCs (C4-C13) and sulphophenyldicarboxylates (SPdCs) (at least C8-C12). Identifications came from high performance liquid chromatography (HPLC) separation, an electrospray interface and mass spectrometry. No evidence for other paths was found. The degradation of LAS in trickling filters inoculated with environmental samples always showed transient SPC intermediates (HPLC) and the presence of the P. lavamentivorans morphotype in the community. One new isolate was obtained. A community able to mineralize LAS contained 4-sulphocatechol-1,2-dioxygenase at high specific activity.
Parvibaculum lavamentivoransT degrades commercial LAS via omega-oxygenation, oxidation and chain shortening through beta-oxidation to yield a wide range of SPCs. The latter are degraded in bacterial communities which contain organisms like P. lavamentivorans, and which utilize sulphocatechol dioxygenase for ring cleavage.
There is one widespread pathway to degrade LAS. Any traces of LAS and larger amounts of SPCs in the effluent from sewage works are exposed to degradative organisms in acclimated and pristine environments. These degradative reactions can now be studied in pure cultures.
旨在测试微小杆菌属嗜洗菌（Parvibaculum lavamentivoransT）是否通过ω-氧化和β-氧化将商业线性烷基苯磺酸盐（LAS）表面活性剂降解为磺基苯基羧酸盐（SPC），该微生物是否广泛存在且可再次分离得到，以及降解群落是否利用4-磺基邻苯二酚1,2-双加氧酶从LAS上裂解芳香环。
异养型微小杆菌属嗜洗菌（Parvibaculum lavamentivoransT）将LAS（侧链长度C10 - C13）转化为SPC（C4 - C13）、α,β-不饱和SPC（C4 - C13）和磺基苯基二羧酸盐（SPdC）（至少C8 - C12）。通过高效液相色谱（HPLC）分离、电喷雾接口和质谱进行鉴定。未发现其他途径的证据。在用环境样品接种的滴滤池中LAS的降解总是显示出瞬时的SPC中间产物（HPLC）以及群落中微小杆菌属嗜洗菌形态型的存在。获得了一株新的分离株。一个能够将LAS矿化的群落含有高比活性的4-磺基邻苯二酚-1,2-双加氧酶。
微小杆菌属嗜洗菌（Parvibaculum lavamentivoransT）通过ω-氧化、氧化以及通过β-氧化进行链缩短来降解商业LAS，从而产生多种SPC。后者在含有微小杆菌属嗜洗菌等微生物的细菌群落中被降解，并且这些群落利用磺基邻苯二酚双加氧酶进行环裂解。
存在一种广泛的降解LAS的途径。污水厂排放物中任何LAS痕迹和大量SPC都会在驯化和原始环境中接触到降解微生物。现在可以在纯培养物中研究这些降解反应。
