精胺和亚精胺在不同培养条件下对NR8383巨噬细胞系的免疫调节作用。
Immunomodulatory action of spermine and spermidine on NR8383 macrophage line in various culture conditions.
作者信息
Pérez-Cano Francisco J, Franch Angels, Castellote Cristina, Castell Margarida
机构信息
Department of Physiology-Division IV, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
出版信息
Cell Immunol. 2003 Dec;226(2):86-94. doi: 10.1016/j.cellimm.2003.09.009.
We examined the effect of spermine (SPM) and spermidine (SPD) on tumor necrosis factor (TNF)alpha and monocyte chemoattractant protein-1 (MCP-1) secretion from macrophages in various culture conditions, including several protocols of polyamines addition and media supplemented with 0, 1 or 15% fetal bovine serum. TNFalpha secretion was inhibited by SPM or SPD added 18h before stimulation in a concentration-dependent manner. Their effect was directly related to the presence of FBS. When SPM or SPD was added simultaneously to the stimulus, the TNFalpha secretion inhibition was higher than that obtained after pre-treatment. In this case, the effect was inversely proportional to the presence of FBS. The addition of polyamines also inhibited the secretion of MCP-1 in NR8383 cells. We conclude that SPM and SPD inhibited the secretion of inflammatory cytokines TNFalpha and MCP-1 in different ways, depending on culture conditions. In any case, SPM was more effective than SPD.
我们研究了在各种培养条件下，包括几种添加多胺的方案以及补充有0%、1%或15%胎牛血清的培养基中，精胺（SPM）和亚精胺（SPD）对巨噬细胞分泌肿瘤坏死因子（TNF）α和单核细胞趋化蛋白-1（MCP-1）的影响。在刺激前18小时添加的SPM或SPD以浓度依赖性方式抑制TNFα分泌。它们的作用与胎牛血清的存在直接相关。当将SPM或SPD与刺激物同时添加时，TNFα分泌的抑制作用高于预处理后获得的抑制作用。在这种情况下，作用与胎牛血清的存在呈反比。多胺的添加也抑制了NR8383细胞中MCP-1的分泌。我们得出结论，根据培养条件，SPM和SPD以不同方式抑制炎性细胞因子TNFα和MCP-1的分泌。在任何情况下，SPM都比SPD更有效。
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