Hassan Mostafa I, Mangoud Amal M, Etewa Samia, Amin Ibrahim, Morsy Tosson A, El Hady Gaber, El Besher Zeinab M, Hammad Kotb
Insect Vectors of Diseases Unit, Faculty of Science, Al Azhar University, Nasr City, Egypt.
J Egypt Soc Parasitol. 2003 Aug;33(2):373-84.
Reverse transcriptase (RT) polymerase chain reaction (PCR) was used to detect hepatitis C Virus (HCV) RNA among heads, guts, larvae and eggs of Culex pipiens complex. The mosquitoes were trapped from homes of hepatitis C patients or among the same organs of symbiotic (normal gut bacteria) and aposymbiotic (without gut bacteria) mosquitoes fed HCV positive blood by an artificial membrane feeder. The eggs and larvae resulted from symbiotic females fed HCV positive blood was tested for HCV-RNA. Hepatitis C virus RNA was detected only in heads of symbiotic mosquitoes collected from homes of HCV positive patients at 3h and 6h after feeding. The virus was detected at 3d and 8d after being fed on HCV-RNA positive blood in guts of the same group. The virus was not detected in the eggs or larvae resulted from female mosquitoes fed on HCV-RNA positive blood. The results raise the possibility of the mechanical and/or biological transmission of HCV by Cx. pipiens, and pave the way to the ongoing study on the effect of gut bacteria of Cx. pipiens in a trial to identify an anti-HCV agent.
采用逆转录酶（RT）聚合酶链反应（PCR）检测致倦库蚊复合体头部、肠道、幼虫和卵中的丙型肝炎病毒（HCV）RNA。这些蚊子是从丙型肝炎患者家中捕获的，或者是通过人工膜饲器给共生（正常肠道细菌）和无共生（无肠道细菌）蚊子喂食HCV阳性血液后，从它们相同器官中捕获的。对喂食HCV阳性血液的共生雌蚊所产的卵和幼虫进行HCV-RNA检测。仅在喂食后3小时和6小时从HCV阳性患者家中采集的共生蚊子头部检测到丙型肝炎病毒RNA。在同一组蚊子喂食HCV-RNA阳性血液后3天和8天，在其肠道中检测到该病毒。在喂食HCV-RNA阳性血液的雌蚊所产的卵或幼虫中未检测到该病毒。这些结果增加了致倦库蚊机械和/或生物传播HCV的可能性，并为正在进行的关于致倦库蚊肠道细菌在鉴定抗HCV药物试验中的作用的研究铺平了道路。
