Chatterjee S N, Pradeep A R
Seribiotech Laboratory, Central Silk Board, Kodathi Campus, Sarjapur Road, Carmelram, Bangalore 560035, Karnataka, India.
Genetika. 2003 Dec;39(12):1612-24.
To identify the molecular markers associated with growth and yield parameters in silkworm, Bombyx mori, RAPD profiles generated with seven UBC primers for fourteen silkworm stocks, originated from China, Japan, India and Russia, were statistically analyzed. Stepwise Multiple Regression Analysis establishes significant association of 45 markers with larval span, growth indices and four cocoon yield parameters relevant for silk production and t-test attest significance of the association of 89.5(1500 bp) and 54.13(300 bp), respectively with longer larval duration and high cocoon weight. The validity of this selection of markers was further supported with Discriminant Function Analysis (DFA) done on the basis of Mahalanobis D2 statistics. The two indices of yield/growth were also tested with DFA, which helped in identifying a few markers and thereby opened scope of using such marker (e.g. 91.11(900 bp)) for incorporating molecular markers in the breeding program for crop improvement in silkworm.
为了鉴定与家蚕生长和产量参数相关的分子标记，对来自中国、日本、印度和俄罗斯的14个家蚕品系用7种UBC引物产生的RAPD图谱进行了统计分析。逐步多元回归分析确定了45个标记与幼虫期、生长指数以及与蚕丝生产相关的4个茧产量参数之间存在显著关联，t检验证明了分别为89.5（1500 bp）和54.13（300 bp）的标记与较长幼虫期和高茧重之间关联的显著性。基于马氏距离D2统计量进行的判别函数分析（DFA）进一步支持了这种标记选择的有效性。还对产量/生长的两个指标进行了DFA测试，这有助于鉴定一些标记，从而为在家蚕作物改良育种计划中纳入分子标记（例如91.11（900 bp））开辟了道路。
