Perepelytsia S I, Koltukova N V, Lysenko T H, Mendzhul M I
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv.
Ukr Biokhim Zh (1999). 2002 Jul-Aug;74(4):109-15.
Aspartokinases have been isolated from the cell-free extracts of Plectonema boryanum, Anabaena variabilis and Synechococcus cedrorum. Their physico-chemical characteristics and peculiarities of retroregulation by amino acids were studied. It has been shown that in P. boryanum cells aspartokinase reaction is catalyzed by only one enzyme, which is similar to the previously described bacterial origin enzymes. Three izoenzymes, which differ in their main properties and amino acids-effectors, have been founded in the cells of A. variabilis. One enzyme with aspartokinase activity, which differs from other cyanobacteria enzymes in some physico-chemical features has been founded in the cells of S. cedrorum.
已从鞘丝藻、多变鱼腥藻和栖热聚球藻的无细胞提取物中分离出天冬氨酸激酶。研究了它们的物理化学特性以及氨基酸对其反馈调节的特点。结果表明，在鞘丝藻细胞中，天冬氨酸激酶反应仅由一种酶催化，该酶与先前描述的细菌来源的酶相似。在多变鱼腥藻细胞中发现了三种同工酶，它们在主要特性和氨基酸效应物方面存在差异。在栖热聚球藻细胞中发现了一种具有天冬氨酸激酶活性的酶，其在某些物理化学特征上与其他蓝藻酶不同。
