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利用信号肽在昆虫细胞中表达和分泌功能性重组单链尿激酶原：抗血管生成素

Expression and secretion of functional recombinant scu-PA:AV in insect cell using signal peptides.

作者信息

Huang Yuhui, Tian Ruiyang, Hu Wei, Wu Xiangfu, Yang Shengli, Gong Yi

机构信息

Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233, PR China.

出版信息

Protein Pept Lett. 2004 Feb;11(1):49-55. doi: 10.2174/0929866043478455.

DOI:10.2174/0929866043478455
PMID:14965279
Abstract

A fusion protein (scu-PA:AV) was expressed in the baculovirus expression system and secreted from Sf9 cells lead by signal peptides, 2mel and 3egt. The scu-PA:AV displays both the urokinase activity and membrane binding activity of its parental components. Our work indicated that it is possible to be developed as a thrombus-targeting drug.

摘要

一种融合蛋白（scu-PA:AV）在杆状病毒表达系统中表达，并由信号肽2mel和3egt引导从Sf9细胞中分泌。scu-PA:AV兼具其亲本组分的尿激酶活性和膜结合活性。我们的研究表明，它有可能被开发成一种血栓靶向药物。

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