通过基质辅助激光解吸电离飞行时间分析揭示的辣椒（辣椒属）基因型间种子蛋白变异。

Seed protein variation among pepper (Capsicum sp.) genotypes revealed by MALDI-TOF analysis.

作者信息

de Pinho Pessoa Filho Marco Aurélio Caldas, Bloch Junior Carlos, da Silva Filho Danilo Fernandes, Galdino Alexsandro Sobreira, da Cunha Rodrigo Maranguape Silva, Alves Maria Aparecida Oliveira, Grangeiro Thalles Barbosa

机构信息

Laboratório de Citogenética e Genética Molecular, Depto de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceará, Av Humberto Monte s/n, 60.451-97, Fortaleza-CE, Brasil.

出版信息

Protein Pept Lett. 2004 Feb;11(1):57-62. doi: 10.2174/0929866043478482.

DOI:10.2174/0929866043478482

PMID:14965280

Abstract

A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccard's coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on on-line databases.

摘要

描述了一种使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析种子蛋白质组的方法。这些数据用于估计12种辣椒（Capsicum）基因型之间的遗传多样性程度。所得光谱被转换为由23个蛋白质数据集组成的二元矩阵，并使用FreeTree软件和杰卡德相似系数计算遗传相似性值。通过在在线数据库上检查其质量，我们还能够鉴定提取物中某些蛋白质的存在。