Nakagawa Masatoshi, Nakanishi Hiroshi
Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1, Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Protein Pept Lett. 2004 Feb;11(1):71-7. doi: 10.2174/0929866043478464.
In the sequence analyses of peptides from nucleoprotein in influenza virus, it was very difficult to obtain the sufficient numbers of fragment ions using the post-source decay (PSD) method in MALDI-TOF-MS. Fluorescent modification of the N-terminal amino group in the target peptides was introduced for the PSD measurement. The fluorescently-labelled peptides gave sufficient fragment ions in the PSD spectrum, which leads to the complete sequence analysis of peptide.
在流感病毒核蛋白肽段的序列分析中，使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）的源后衰变（PSD）方法很难获得足够数量的碎片离子。为了进行PSD测量，对目标肽段的N端氨基进行了荧光修饰。荧光标记的肽段在PSD谱图中给出了足够的碎片离子，从而实现了肽段的完整序列分析。
