Matsushima Y, Makino H, Kanatsuka A, Yamamoto M, Kumagai A
Endocrinol Jpn. 1978 Apr;25(2):111-5. doi: 10.1507/endocrj1954.25.111.
By the enzyme-labeled antibody method, cells containing somatostatin (SRIF) as well as insulin or glucagon were identified in pancreatic islets of the rat. SRIF antiserum was raised in rabbits followin immunization with synthetic SRIF coupled with human serum alpha-globulin and did not cross-react with hypothalamic, pituitary or gastrointestinal hormones using our immunoassay method. In the control rats, SRIF-containing cells were scattered in the periphery of the islets in close proximity to the outer glucagon containing cells. These latter cells were distributed in the outermost periphery of the islets. Insulin-containing cells were located in the central portion of the islets and dominantly occupied most of the islet. In the streptozotocin-diabetic rat, SRIF-containing cells were significantly increased in number whereas insulin-containing cells were markedly reduced. It is suggested from these findings that the number as well as the distribution of SRIF-, insulin- and glucagon-containing cells was important to the physiological and pathophysiological functioning of the islet.
通过酶标抗体法，在大鼠胰岛中鉴定出含有生长抑素（SRIF）以及胰岛素或胰高血糖素的细胞。用与人类血清α-球蛋白偶联的合成SRIF免疫家兔后产生了SRIF抗血清，并且使用我们的免疫测定方法，该抗血清与下丘脑、垂体或胃肠激素无交叉反应。在对照大鼠中，含SRIF的细胞散在于胰岛周边，紧邻含胰高血糖素的外层细胞。后者分布在胰岛的最外层周边。含胰岛素的细胞位于胰岛中央部分，占胰岛的大部分。在链脲佐菌素诱导的糖尿病大鼠中，含SRIF的细胞数量显著增加，而含胰岛素的细胞明显减少。从这些发现表明，含SRIF、胰岛素和胰高血糖素的细胞数量以及分布对胰岛的生理和病理生理功能很重要。
