Arduini I, Godbold D L, Onnis A
Dipartimento di Agronomia e Gestione dell'Agro-Ecosistema, Università degli Studi Pisa, Via S. Michele degli Scalzi 2, 56124 Pisa, Italy.
Tree Physiol. 1995 Jun;15(6):411-5. doi: 10.1093/treephys/15.6.411.
We assessed the effects of Cu on root growth and morphology of stone pine (Pinus pinea L.) and maritime pine (Pinus pinaster Ait.) seedlings grown in culture solutions supplied with 0.012 (control), 0.1, 1 or 5 micro M CuSO(4). The presence of 5 micro M Cu in the nutrient solution completely inhibited root growth of both species within 3 days. In both species, taproot elongation was reduced in the presence of 1 micro M Cu, although partial growth recovery occurred after 7 days of treatment. The presence of 0.1 micro M Cu in the culture solution slightly enhanced root elongation in P. pinaster, but did not significantly influence root elongation in P. pinea. In both species, root weight per unit length increased in response to Cu exposure, and in P. pinaster, root diameter was significantly increased. The Cu treatments also affected lateral root number and length. In the presence of 1 micro M Cu, both species formed only short lateral primordia. The 1 micro M Cu treatment increased the lateral root index (number of roots per cm of root length) of P. pinaster, but decreased that of P. pinea, compared with control values. Neither the 0.1 nor 1 micro M Cu treatment had a significant effect on the mitotic index of either species. We conclude that cell elongation is more sensitive to Cu than cell division. Cell membrane damage, as indicated by Trypan blue staining, occurred after 10 days of exposure to 1 micro M Cu.
我们评估了铜对在添加了0.012（对照）、0.1、1或5微摩尔硫酸铜的培养液中生长的意大利石松（Pinus pinea L.）和海岸松（Pinus pinaster Ait.）幼苗根系生长和形态的影响。营养液中5微摩尔铜的存在在3天内完全抑制了两个物种的根系生长。在两个物种中，1微摩尔铜的存在会降低主根伸长，不过处理7天后部分生长恢复。培养液中0.1微摩尔铜的存在略微增强了海岸松的根伸长，但对意大利石松的根伸长没有显著影响。在两个物种中，单位长度根重随铜暴露而增加，在海岸松中，根直径显著增加。铜处理也影响侧根数量和长度。在1微摩尔铜存在下，两个物种都只形成短的侧根原基。与对照值相比，1微摩尔铜处理增加了海岸松的侧根指数（每厘米根长的根数），但降低了意大利石松的侧根指数。0.1微摩尔和1微摩尔铜处理对两个物种的有丝分裂指数均无显著影响。我们得出结论，细胞伸长对铜比细胞分裂更敏感。用台盼蓝染色表明，暴露于1微摩尔铜10天后发生了细胞膜损伤。
