Beyeler W, Bachofen R
Eur J Biochem. 1978 Jul 17;88(1):61-7. doi: 10.1111/j.1432-1033.1978.tb12422.x.
The kinetics of 32Pi incorporation into adenine nucleotides by subchloroplast particles in the light is studied with a continuous flow apparatus allowing measurements between 3 and 200 ms. After a short lag time from 1 to 3 ms ATP synthesis proceeds with a constant rate. During the first few milliseconds a faster labelling of ADP is detected. This labelling of ADP reaches a constant level up to 1 molecule ADP labelled per molecule of coupling factor present. The labelling pattern in ATP indicates that the labelled ADP does not equilibrate with free ADP. The addition of 32Pi to a phosphorylating system during the light phase (32Pi pulse) exhibits unchanged kinetic characteristics for labelling of ATP and ADP. These results indicate a phosphorylation of AMP to ADP being an intermediate step in photophosphorylation. In experiments carried out in the dark no label is found in ATP within the time analysed. However the labelling of ADP occurs in the same way as in the light.
利用连续流动装置研究了亚叶绿体颗粒在光照下将³²Pi掺入腺嘌呤核苷酸的动力学，该装置可在3至200毫秒之间进行测量。在1至3毫秒的短暂延迟时间后，ATP合成以恒定速率进行。在最初的几毫秒内，检测到ADP的标记速度更快。这种ADP的标记达到恒定水平，每存在一个耦合因子分子就有一个ADP分子被标记。ATP中的标记模式表明，标记的ADP不会与游离ADP达到平衡。在光照阶段向磷酸化系统中添加³²Pi（³²Pi脉冲）时，ATP和ADP标记的动力学特征不变。这些结果表明，AMP磷酸化为ADP是光磷酸化的中间步骤。在黑暗中进行的实验中，在所分析的时间内ATP中未发现标记。然而，ADP的标记方式与光照下相同。
