Vinkler C, Rosen G, Boyer P D
J Biol Chem. 1978 Apr 25;253(8):2507-10.
The labeling of ATP and ADP in the first few milliseconds of exposure of chloroplast thylakoids to light, 32Pi, and ADP has been measured. At least 4 mol of ATP/mol of coupling factor 1 (CF1)-ATPase can be formed without detectable labeling of membrane-bound or free ADP. Such results are consistent with ADP and not AMP as the primary acceptor of Pi in photophosphorylation. Evidence is presented demonstrating that quenching with perchloric acid, as used in these and earlier experiments, is satisfactory for the measurement of the amount and nature of membrane-bound nucleotides.
已对叶绿体类囊体在光照、32Pi 和 ADP 存在下最初几毫秒内 ATP 和 ADP 的标记情况进行了测定。在未检测到膜结合或游离 ADP 有明显标记的情况下，每摩尔偶联因子 1（CF1）-ATP 酶至少可形成 4 摩尔 ATP。这些结果与 ADP 而非 AMP 作为光合磷酸化中 Pi 的主要受体相一致。文中给出的证据表明，在这些及早期实验中所使用的高氯酸淬灭法，对于测定膜结合核苷酸的量和性质而言是令人满意的。
