[顺铂耐药细胞系Tca8113/CDDP的建立及耐药相关基因表达]
[Establishment and drug-resistance gene expression of cisplatin-resistant cell line Tca8113/CDDP].
作者信息
Zhou Xiao-jian, Li Qin, Chen Wan-tao
机构信息
Department of Oral Maxillofacial Surgery, School of Stomatology, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2003 Dec;12(6):432-5.
OBJECTIVE
To establish a CDDP-resistant cell line from human tongue squamous cell carcinoma cell line Tca8113 and to compare the drug-resistance gene expression between them.
METHODS
The concentration of CDDP added to Tca8113 cells was increased stepwise to induce the CDDP-resistance. The index of drug-resistance was estimated by MTT assay. The expression of MDR-1, MRP, GST-pi and Bcl-2 was measured by RT-PCR optical density OD.
RESULTS
The CDDP-resistant cell line, Tca8113/CDDP was established, whose drug-resistance index was 9.2. The expression of MDR-1, MRP, cyclin D1 and Bcl-2 increased respectively.
CONCLUSION
The expression of drug-resistance gene in Tca8113/CDDP was significantly higher than that in Tca8113.
目的
从人舌鳞状细胞癌细胞系Tca8113建立顺铂耐药细胞系，并比较两者之间的耐药基因表达。
方法
逐步增加添加到Tca8113细胞中的顺铂浓度以诱导顺铂耐药。通过MTT法评估耐药指数。通过RT-PCR光密度OD测量多药耐药基因1（MDR-1）、多药耐药相关蛋白（MRP）、谷胱甘肽S转移酶π（GST-pi）和Bcl-2的表达。
结果
建立了顺铂耐药细胞系Tca8113/CDDP，其耐药指数为9.2。MDR-1、MRP、细胞周期蛋白D1（cyclin D1）和Bcl-2的表达分别增加。
结论
Tca8113/CDDP中耐药基因的表达明显高于Tca8113。
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