Clark T Nicole, White Catherine A, Bartlett Michael G
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, USA.
Rapid Commun Mass Spectrom. 2004;18(4):405-11. doi: 10.1002/rcm.1329.
A rapid and efficient high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for the determination of concentrations of the carbocyclic nucleoside antiviral Abacavir in maternal rat plasma, amniotic fluid, placental and fetal tissue samples has been developed and validated. All tissue samples were homogenized in water prior to analysis and all samples were prepared by acetonitrile precipitation followed by dilution with HPLC-grade water. Separation of the analyte and internal standard from the matrices was achieved on a C(8) analytical column (2.1 x 150 mm, 5 microm particle size). The mobile phase consisted of 10 mM ammonium acetate/acetonitrile using a gradient method at a flow rate of 0.25 mL/min for all matrices. The method yields retention times of approximately 3.4 and 5.1 min for the internal standard (Azidouridine) and Abacavir, respectively. For all matrices the limit of detection was approximately 1 ng/ml. Recoveries from the different matrices ranged from 53-87% for Abacavir and from 69-84% for Azidouridine. Within- and between-run precision (%RSD) and accuracy (%Error) were under 15% for all matrices.
已开发并验证了一种快速高效的高效液相色谱/串联质谱（HPLC/MS/MS）方法，用于测定母鼠血浆、羊水、胎盘和胎儿组织样本中碳环核苷类抗病毒药物阿巴卡韦的浓度。所有组织样本在分析前均在水中匀浆，所有样本均通过乙腈沉淀，然后用HPLC级水稀释来制备。在C(8)分析柱（2.1×150 mm，5微米粒径）上实现了分析物和内标与基质的分离。流动相由10 mM醋酸铵/乙腈组成，采用梯度法，所有基质的流速均为0.25 mL/min。该方法测得内标（叠氮尿苷）和阿巴卡韦的保留时间分别约为3.4分钟和5.1分钟。对于所有基质，检测限约为1 ng/ml。阿巴卡韦在不同基质中的回收率为53 - 87%，叠氮尿苷为69 - 84%。所有基质的批内和批间精密度（%RSD）和准确度（%误差）均在15%以下。
