Song Hai, Xia Shuang-Luo, Liao Cheng, Li Yi-Liang, Wang Yi-Fei, Li Tsai-Ping, Zhao Mu-Jun
P.O. Box 35, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
World J Gastroenterol. 2004 Feb 15;10(4):509-13. doi: 10.3748/wjg.v10.i4.509.
To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray.
The (33 )P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR.
Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis.
cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma.
利用cDNA微阵列从原发性肿瘤样本的差异表达基因中揭示新的肿瘤标志物和靶基因。
从同一患者的肝癌组织和相邻的非癌肝组织的信使RNA逆转录合成（33）P标记的cDNA，并用于与包含8400个已知且独特的人类cDNA基因靶点的LifeGrid 1.0 cDNA微阵列印迹杂交，从而在一对人类肝脏肿瘤组织中产生基因表达谱。在对8400个基因的基因表达进行全面分析后，我们选择了一些基因，使用Northern印迹和RT-PCR来确认差异表达。
对杂交信号的平行分析使我们能够获得基因表达谱，其中约500个基因在配对的肝脏肿瘤组织中差异表达。我们鉴定出4个基因，其中3个（Beclin 1、RbAp48和Pir51）在肝脏肿瘤组织中的表达增加，1个（醛缩酶b）表达降低。此外，RT-PCR分析也显示了这些基因在6种肝癌细胞系中的表达。
cDNA微阵列可高通量鉴定基因表达的变化。首次报道了编码Beclin 1、RbAp48、Pir51和醛缩酶b的基因可能与肝癌有关。
