Zhang Shu-Hui, Cong Wen-Ming, Shi Jing-Quan, Wei Hong
Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
World J Gastroenterol. 2004 Feb 15;10(4):521-4. doi: 10.3748/wjg.v10.i4.521.
To investigate the frequency of genomic instability in murine hepatocellular carcinoma (HCC) cell lines Hca/A2-P(P) and Hca/163-F(F) with low and high metastatic capacity, and to explore its association with the occurrence and metastasis of hepatocellular carcinomas.
Forty microsatellite markers were randomly selected to examine P and F cells for genomic instability using PCR-simple sequence length polymorphism (PCR-SSLP) analysis.
Allelic genes on the chromosomes of P cell line with thirty informative microsatellite loci were paralleled to those of inbred strain C(3)H mouse, while those of F cell line with 28 loci were paralleled to those of inbred strain C(3)H mice. The frequency of microsatellite alterations was 37.5% and 42.5% in P cell line and F cell line, respectively. There were different alterations of allelic band 9 at loci between P and F cells, among which, the frequency of microsatellite alterations was most commonly seen on chromosomes 3, 7, 11 and 16.
Genomic instability in mouse chromosomes 3, 7, 11 and 16 may play a more important role in the development and progression of HCC in mice. It is suggested that these two sub-clones derived from a same hepatic tumor in homozygous mouse present different genetic features.
研究低转移能力的小鼠肝癌（HCC）细胞系Hca/A2-P（P）和高转移能力的Hca/163-F（F）中基因组不稳定性的频率，并探讨其与肝细胞癌发生和转移的关系。
随机选择40个微卫星标记，采用聚合酶链反应-简单序列长度多态性（PCR-SSLP）分析检测P和F细胞的基因组不稳定性。
具有30个信息性微卫星位点的P细胞系染色体上等位基因与近交系C(3)H小鼠的等位基因平行，而具有28个位点的F细胞系染色体上等位基因与近交系C(3)H小鼠的等位基因平行。P细胞系和F细胞系中微卫星改变的频率分别为37.5%和42.5%。P和F细胞在基因座9处等位基因带存在不同改变，其中微卫星改变频率在3号、7号、11号和16号染色体上最为常见。
小鼠3号、7号、11号和16号染色体上的基因组不稳定性可能在小鼠肝癌的发生和发展中起更重要的作用。提示来自纯合小鼠同一肝肿瘤的这两个亚克隆具有不同的遗传特征。
