Dennison Christopher, Sato Katsuko
School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK.
Inorg Chem. 2004 Feb 23;43(4):1502-10. doi: 10.1021/ic034861v.
The native type 1 copper ion of spinach plastocyanin has been substituted with Co(II). The UV/vis spectrum of this derivative is similar to those for other Co(II)-substituted cupredoxins. The paramagnetic 1H NMR spectrum of Co(II) plastocyanin has been completely assigned. A number of similar studies on Co(II) cupredoxins have been published, but this is the first such analysis of a substituted plastocyanin that possesses the archetypal type 1 active site. A truly representative comparison of the available paramagnetic 1H NMR data for Co(II) cupredoxins is now possible. We demonstrate in this work that there is very little difference in the metal-ligand contacts between the Co(II) derivatives of cupredoxins possessing a type 1 axial site (plastocyanin) and those having perturbed (rhombic) spectroscopic features.
菠菜质体蓝素的天然I型铜离子已被Co(II)取代。该衍生物的紫外/可见光谱与其他Co(II)取代的铜氧化还原蛋白的光谱相似。Co(II)质体蓝素的顺磁1H NMR谱已被完全归属。关于Co(II)铜氧化还原蛋白已经发表了许多类似的研究，但这是对具有原型I型活性位点的取代质体蓝素的首次此类分析。现在可以对Co(II)铜氧化还原蛋白的现有顺磁1H NMR数据进行真正有代表性的比较。我们在这项工作中证明，具有I型轴向位点的铜氧化还原蛋白（质体蓝素）的Co(II)衍生物与具有扰动（菱形）光谱特征的Co(II)衍生物之间，金属-配体接触几乎没有差异。
