Andersson B
The Forestry Research Institute of Sweden, PO Box 3, S-910 36 Sävar, Sweden.
Tree Physiol. 1994 Mar;14(3):313-22. doi: 10.1093/treephys/14.3.313.
Full-sib families of Pinus sylvestris (L.) from genetically identical parent clones, grown at three geographic locations (64, 59.5, and 56 degrees N), were tested for autumn frost hardiness. At one of the locations (64 degrees N), maternal clones were grown in plots subjected to different site treatments. Progenies raised in a heated greenhouse with additional artificial light were induced to undergo two shoot elongation periods, separated by a period with long nights and non-hardening temperatures, followed by a hardening period. Frost hardiness was tested by exposing seedlings to -17, -19, or -21 degrees C during the hardening period. Progenies produced at the northernmost locality (64 degrees N) were the most hardy. The hardiness ranking among localities and the magnitude of differences in hardiness were in accordance with earlier results obtained from the same crosses that had undergone only one shoot elongation period. Effects of maternal site treatments were significant but small compared with family differences. The growth regimes used in the present study did not eliminate maternal effects induced by geographic location, but maternal effects related to site treatment decreased substantially in relation to family variation when compared with seedlings that had undergone only a single shoot elongation period. The results are discussed in relation to other studies of the same crosses raised under different growth conditions.
对来自基因相同的亲本克隆的欧洲赤松（Pinus sylvestris (L.)）全同胞家系在三个地理位置（北纬64度、59.5度和56度）进行了秋季抗冻性测试。在其中一个地点（北纬64度），母本克隆种植在经过不同立地处理的地块中。在有加温及额外人工光照的温室中培育的子代经历了两个新梢伸长阶段，中间间隔一个长夜和非硬化温度阶段，随后是一个硬化阶段。在硬化阶段，通过将幼苗暴露在-17℃、-19℃或-21℃来测试抗冻性。最北端地点（北纬64度）产生的子代最抗冻。各地点间的抗冻性排名以及抗冻性差异的大小与之前对同一杂交组合仅经历一个新梢伸长阶段所获得的结果一致。母本立地处理的影响显著，但与家系差异相比很小。本研究中使用的生长方式并未消除地理位置诱导的母本效应，但与仅经历单个新梢伸长阶段的幼苗相比，与立地处理相关的母本效应相对于家系变异大幅降低。结合在不同生长条件下培育的同一杂交组合的其他研究对结果进行了讨论。
