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肝片吸虫:通过全身蛋白质等电聚焦条带模式对韩国和美国的吸虫进行比较

Fasciola hepatica: comparison of flukes from Korea and the United States by isoelectric focusing banding patterns of whole-body protein.

作者信息

Lee C G, Zimmerman G L, Wee S H

机构信息

College of Veterinary Medicine, Oregon State University, Corvallis 97331.

出版信息

Vet Parasitol. 1992 May;42(3-4):311-6. doi: 10.1016/0304-4017(92)90073-i.

Abstract

Comparisons were made between the flukes from Chonnam, Korea and Oregon, USA by isoelectric focusing (IEF) of whole-body protein. Adult Fasciola hepatica were recovered from bile ducts of Korean native cattle. Whole-body protein of the flukes was subjected to IEF, and the banding patterns of the fluke protein were compared with those of North American F. hepatica recovered from experimentally infected calves. The overall banding pattern of F. hepatica from Korea was essentially identical to that of F. hepatica from the United States. These results provide further support for the usefulness of this technique in differentiating Fasciola species in other geographical areas.

摘要

通过对虫体全蛋白进行等电聚焦(IEF),比较了来自韩国全罗南道和美国俄勒冈州的吸虫。从韩国本地牛的胆管中采集到成年肝片吸虫。对吸虫的全蛋白进行等电聚焦,并将吸虫蛋白的条带模式与从实验感染小牛中采集到的北美肝片吸虫的条带模式进行比较。来自韩国的肝片吸虫的总体条带模式与来自美国的肝片吸虫基本相同。这些结果为该技术在区分其他地理区域的片形吸虫种类方面的实用性提供了进一步的支持。

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