Kerlin R L, Allingham P G
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Qld., Australia.
Vet Parasitol. 1992 Jun;43(1-2):115-29. doi: 10.1016/0304-4017(92)90054-d.
Naturally acquired immunity to buffalo fly (Haematobia irritans exigua) infestation was examined in cattle. Animals exposed to flies had serum antibodies to buffalo fly antigens at levels that correlated with the intensity of exposure. Two weeks of intense exposure to buffalo fly induced an increase in peripheral blood eosinophil numbers and a concomitant rise in serum antibody levels in exposed animals. Antigens specific for antibody induced by natural exposure were identified using antisera from exposed cattle to probe Western blots of whole fly homogenate separated using SDS-PAGE. Similar immunoreactive bands were found with buffalo fly saliva. Immunoreactive proteins were partially purified from whole fly homogenates by anion-exchange chromatography. Fractions eluted from columns were screened using Western blots probed with serum from exposed animals. Exposed animals showed immediate hypersensitivity to partially purified antigens and to buffalo fly saliva. Flies which fed on exposed animals with high serum levels of antibody to fly antigens did not show greater mortality than flies fed on unexposed animals.
对牛群中自然获得的抗水牛蝇（嗜人血蝇）感染免疫力进行了研究。接触过苍蝇的动物体内针对水牛蝇抗原的血清抗体水平与接触强度相关。两周的强烈水牛蝇接触使接触组动物外周血嗜酸性粒细胞数量增加，同时血清抗体水平升高。用接触组牛的抗血清探测经SDS - PAGE分离的全蝇匀浆的蛋白质印迹，以鉴定自然接触诱导产生的抗体的特异性抗原。在水牛蝇唾液中发现了类似的免疫反应条带。通过阴离子交换色谱从全蝇匀浆中部分纯化免疫反应蛋白。用接触组动物的血清探测蛋白质印迹，对从柱上洗脱的组分进行筛选。接触组动物对部分纯化的抗原和水牛蝇唾液表现出速发型超敏反应。以血清中针对蝇抗原抗体水平高的接触组动物为食的苍蝇，其死亡率并不高于以未接触组动物为食的苍蝇。
