Linke H A, Moss S J
Department of Microbiology, New York University Dental Center.
Z Ernahrungswiss. 1992 Jun;31(2):147-54. doi: 10.1007/BF01623073.
A sensitive high performance liquid chromatography (HPLC) assay was developed for the qualitative and quantitative determination of carbohydrate sweeteners and organic acids in oral fluid. To separate these compounds, an ion-moderated partition resin HPLC column (Aminex HPX-87H) was used. All components of the HPLC system were interconnected using stainless steel capillary tubing. Isocratic elution with 0.01N sulfuric acid provided the profile of both compound classes. The compounds were detected using a refractive index detector. The method employed computerized data collection and integration (Omega-2 system) with a detection sensitivity of 0.1 micrograms compound per HPLC assay (80 microliters). This method is useful in caries research, because it detects minute amounts of sugars and organic acids in oral fluid during clearance studies of various foods in the mouth.
开发了一种灵敏的高效液相色谱（HPLC）分析法，用于定性和定量测定口腔液中的碳水化合物甜味剂和有机酸。为了分离这些化合物，使用了离子调节分配树脂HPLC柱（Aminex HPX-87H）。HPLC系统的所有组件都使用不锈钢毛细管相互连接。用0.01N硫酸等度洗脱可得到这两类化合物的图谱。使用折光率检测器检测这些化合物。该方法采用计算机化数据采集和积分（Omega-2系统），每次HPLC分析（80微升）的检测灵敏度为0.1微克化合物。该方法在龋齿研究中很有用，因为它能在口腔中各种食物的清除研究期间检测出口腔液中微量的糖和有机酸。
