Paiva Sandra, Devaux Frederic, Barbosa Sonia, Jacq Claude, Casal Margarida
Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, 4710-057 Braga Codex, Portugal.
Yeast. 2004 Feb;21(3):201-10. doi: 10.1002/yea.1056.
To identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2Delta strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate.
为了鉴定酿酒酵母中参与乙酸摄取的新基因，我们对从葡萄糖转移至乙酸的细胞进行了基因表达谱分析。观察到酵母适应不良非发酵碳源时的基因表达重编程，包括显著的代谢变化、翻译机器的整体激活、线粒体生物合成以及已知或假定转运蛋白的诱导。其中，基因ADY2/YCR010c被鉴定为乙酸转运的新关键元件，它与解脂耶氏酵母GPR1基因同源，该基因在乙酸敏感性中起作用。酿酒酵母中ADY2的破坏消除了乙酸的主动转运。ady2Δ菌株的微阵列分析表明，该基因不是乙酸应答的关键调节因子，其作用与乙酸转运直接相关。Ady2p预计是一种膜蛋白，是一个有价值的乙酸转运蛋白候选物。
