Amino Nobuyuki, Kimura Masahiro, Tanaka Susumu, Izumi Yukiko, Ikemoto Masaki, Tatsumi Ke-ita, Takano Toru, Hidaka Yoh
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871.
Rinsho Byori. 2004 Jan;52(1):44-50.
Liver dysfunction has been found in 8.1% of postpartum women in the general population. This dysfunction was speculated to be developed by postpartum aggravation of subclinical autoimmune hepatitis. Therefore, we developed two methods for detection of autoantibodies to liver-specific antigens: an ELISA for anti-liver-specific arginase antibodies, and a highly sensitive radioligand assay for anti-CYP2D6 antibodies. Basic examinations of dilution curve, inhibition study and reproducibility were satisfactory for clinical application in both assays. Anti-arginase antibodies and anti-CYP2D6 antibodies were found in 28.6% and 42.6% of patients with autoimmune hepatitis, respectively. There was no correlation between the two autoantibodies and thus, combined use of these antibodies detects 55.3% of autoimmune hepatitis. Autoimmune hepatitis exists frequently when we include mild cases.
在普通人群中，8.1%的产后女性存在肝功能障碍。这种功能障碍被推测是由亚临床自身免疫性肝炎的产后加重所致。因此，我们开发了两种检测肝脏特异性抗原自身抗体的方法：一种用于检测抗肝脏特异性精氨酸酶抗体的酶联免疫吸附测定（ELISA），以及一种用于检测抗CYP2D6抗体的高灵敏度放射性配体测定法。两种测定法的稀释曲线、抑制研究和重现性的基础检测对于临床应用而言均令人满意。自身免疫性肝炎患者中，分别有28.6%和42.6%检测出抗精氨酸酶抗体和抗CYP2D6抗体。这两种自身抗体之间无相关性，因此，联合使用这些抗体可检测出55.3%的自身免疫性肝炎。若纳入轻症病例，自身免疫性肝炎较为常见。
